Quantitative PCR (qPCR) is a highly sensitive molecular technique used to detect and quantify nucleic acids —including DNA,mRNA,microRNA,and long non-coding RNA (lncRNA).
\nWhen combined with a reverse transcription step (RT-qPCR),it becomes the gold standard for targeted gene expression analysis. qPCR offers excellent sensitivity,specificity,and a wide linear dynamic range,making it well suited for both research and clinical trial applications.
"},{"faq_question":"Do you offer off-the-shelf assays? ","faq_answer":"Yes. CellCarta offers access to a broad menu of validated off-the-shelf (OTS) qPCR assays,including the AmoyDx Pan Lung Cancer Panel,Biofidelity Aspyre®Lung assay,Cobas mutation tests (EGFR,KRAS,BRAF V600,PIK3CA,BRAF/NRAS),and Qiagen Therascreen kits (EGFR,PIK3CA,KRAS,FGFR).
\nThese widely accepted assays support patient selection and identification of druggable targets. View our genomic assay list for a complete overview.
"},{"faq_question":"What sample types does CellCarta accept for qPCR applications? ","faq_answer":"CellCarta's qPCR services are equipped to process a broad range of sample types,including FFPE (formalin-fixed paraffin-embedded) tissue,liquid biopsies,whole blood,and cell lysates.
\nOur team has processed tens of thousands of clinical samples across these formats,with validated workflows designed to maintain data integrity even from challenging or limited-input specimens.
"},{"faq_question":"What qPCR platforms do you use for analysis?","faq_answer":"CellCarta operates multiple leading qPCR instrument platforms to ensure flexibility and fit-for-purpose analysis. Our team works with the cobas system (Roche),Rotor-Gene Q (Qiagen),Idylla (Biocartis),and QuantStudio (ThermoFisher).
\nThis multi-platform infrastructure supports both off-the-shelf assays and custom-designed,validated workflows tailored to your study requirements.
"}]}#-supreme-cellcarta-featured-resources-16-144{cards-data:[{"image_url":"\/wp-content\/uploads\/2026\/05\/webinar.jpg","card_type":"Webinar","date":"Feb 12","reading_time":"Reading Time - 5 minutes","title":"Resources Title Goes Here","description":"Lorem ipsum dolor sit amet,consectetuer adipiscing elit. Aenean commodo ligula eget dolor.","url":"#"},{"image_url":"\/wp-content\/uploads\/2026\/05\/webinar-2.jpg","card_type":"Webinar","date":"Feb 12","reading_time":"Reading Time - 5 minutes","title":"Resources Title Goes Here","description":"Lorem ipsum dolor sit amet,consectetuer adipiscing elit. Aenean commodo ligula eget dolor.","url":"#"},{"image_url":"\/wp-content\/uploads\/2026\/05\/webinar-3.jpg","card_type":"Webinar","date":"Feb 12","reading_time":"Reading Time - 5 minutes","title":"Resources Title Goes Here","description":"Lorem ipsum dolor sit amet,consectetuer adipiscing elit. Aenean commodo ligula eget dolor.","url":"#"}]}#-supreme-cellcarta-service-blocks-45-176{blocks-data:[{"image":"https://cellcarta.com/wp-content/uploads/2026/07/CellCarta-799-scaled.jpg","image_alt":"","title":"Sequencing Services ","description":"In-depth genomics and transcriptomics data for your translational and clinical applications. ","button_text":"Discover","button_url":"/capabilities/genomics/sequencing-services/","button_target":"_self"},{"image":"https://cellcarta.com/wp-content/uploads/2026/07/CellCarta-525-1-scaled.jpg","image_alt":"","title":"Digital PCR (dPCR)","description":"Nucleic acid quantification with superior accuracy,precision,and sensitivity. ","button_text":"Discover","button_url":"/capabilities/genomics/digital-pcr-services/","button_target":"_self"},{"image":"https://cellcarta.com/wp-content/uploads/2026/06/Assays-by-Mutations-10-IMG_2181.jpg","image_alt":"","title":"Assays by Mutations ","description":"Robust detection of key oncogenes,including EGFR,KRAS,BRAF,FGFR,and PI3KCA. ","button_text":"Discover","button_url":"/capabilities/genomics/assays-by-mutations/","button_target":"_self"}]}#-supreme-cellcarta-tab-content-11-144{tabs-data:[{"tab_title":"Validated OTS Assays ","content_title":"","content_body":"CellCarta can support any qPCR-based application,with experience running key OTS assays for AGA assessment. Our portfolio includes:
\nDownload our brochure for more details on the genomics assays available at CellCarta.
","tab_image":"https://cellcarta.com/wp-content/uploads/2026/06/Validated-OTS-Assays-tab-111.jpg","image_alt":""},{"tab_title":"Custom Developments ","content_title":"","content_body":"Our team has expertise in the design and validation of custom primers/probe sets and overall assay design for AGA detection. Our solutions will fully support qPCR studies from discovery and exploration all the way through to patient management and CDx.
\nWith access to over 5000 validated,phenotype-characterized samples,our team can reduce assay development timelines by 4-6 weeks,accelerating your research progress.
\nContact us about any custom developments.
","tab_image":"https://cellcarta.com/wp-content/uploads/2026/06/Custom-Developments-tab-119.jpg","image_alt":""},{"tab_title":"High-Throughput Workflows ","content_title":"","content_body":"Our team has proficiency on several key platforms for the high-throughput and rigorous testing of clinical samples,including:
\nOur platforms are validated for all biological matrices,including both tissue and liquid biopsies.
\nWith experience performing qPCR on tens of thousands of samples,our workflows can handle high-throughput processing with ease.
","tab_image":"https://cellcarta.com/wp-content/uploads/2026/06/High-throughput-Workflows-tab-149.jpg","image_alt":""}]}