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Flow Cytometry Services

Precise, reproducible flow cytometry solutions for robust phenotypical and functional analyses of your cell populations.

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Scientist operating flow cytometry instrument in CAP/CLIA-accredited laboratory

A Complete Flow Cytometry Platform

Characterize the full range of immune cell populations at the single-cell level with conventional and spectral flow cytometry solutions.

We operate a variety of leading flow platforms, with advanced data analytics to extract meaningful insight from complex datasets. Our scientists combine deep flow cytometry and immunology expertise to guide assay design, study planning, and data analysis.

All flow cytometry services are delivered in a GCLP environment across CAP-accredited, CLIA-certified laboratories.

Built for Complex Clinical Programs

Run studies using multiple advanced platforms, including:

  • BD Lyric™
  • BD LSRFortessa™
  • Cytek™ Aurora
  • BD FACSAria II™
  • CyTOF®

Our flow cytometry services can be tailored to your study’s requirements. Whether we leverage the built-in audit trail of the BD FACSLyric for higher regulatory endpoints, or the high-parameter capability of the spectral Cytek Aurora platform, all depend on the specific needs of your program.

Our flow cytometry services can support a  vast range of clinical samples, including:

  • Whole blood
  • PBMC (fresh or cryopreserved)
  • Bone Marrow aspirate (BMA)
  • Fine-needle aspiration (FNA) biopsies
  • Cerebrospinal fluid (CSF) samples
  • Dissociated tumors/tissue
  • Cell lines

Our team can provide guidance on sample stability and logistics, and offer in-house sample procurement, preparation, stimulation, or stabilization if needed.

Select from our pre-validated catalogue of panels for rapid flow implementation, or collaborate with our scientists to design a fully custom panels for a wide range of clinical immune monitoring endpoints.

Custom solutions can include:

  • T-cell exhaustion profiling
  • Immune checkpoint assessment
  • BCMA monitoring, cytotoxicity markers
  • Helper T-cell polarization

Obtain fast, reliable results from complex flow cytometry datasets with CellEngine®, CellCarta's proprietary, cloud-based analysis software. Machine learning-assisted autogating reduces manual analysis time, metadata-driven workflows minimize human error, and supported UMAP/FlowSOM clustering can run in minutes, all within a 21 CFR 11-compliant environment.

Run studies using multiple advanced platforms, including:

  • BD Lyric™
  • BD LSRFortessa™
  • Cytek™ Aurora
  • BD FACSAria II™
  • CyTOF®

Our flow cytometry services can be tailored to your study’s requirements. Whether we leverage the built-in audit trail of the BD FACSLyric for higher regulatory endpoints, or the high-parameter capability of the spectral Cytek Aurora platform, all depend on the specific needs of your program.

BD Lyric and Cytek Aurora flow cytometry instruments in CellCarta laboratory

Our flow cytometry services can support a  vast range of clinical samples, including:

  • Whole blood
  • PBMC (fresh or cryopreserved)
  • Bone Marrow aspirate (BMA)
  • Fine-needle aspiration (FNA) biopsies
  • Cerebrospinal fluid (CSF) samples
  • Dissociated tumors/tissue
  • Cell lines

Our team can provide guidance on sample stability and logistics, and offer in-house sample procurement, preparation, stimulation, or stabilization if needed.

Scientist preparing diverse clinical sample types for flow cytometry analysis

Select from our pre-validated catalogue of panels for rapid flow implementation, or collaborate with our scientists to design a fully custom panels for a wide range of clinical immune monitoring endpoints.

Custom solutions can include:

  • T-cell exhaustion profiling
  • Immune checkpoint assessment
  • BCMA monitoring, cytotoxicity markers
  • Helper T-cell polarization
Scientist selecting customizable flow cytometry panel for clinical immune monitoring

Obtain fast, reliable results from complex flow cytometry datasets with CellEngine®, CellCarta's proprietary, cloud-based analysis software. Machine learning-assisted autogating reduces manual analysis time, metadata-driven workflows minimize human error, and supported UMAP/FlowSOM clustering can run in minutes, all within a 21 CFR 11-compliant environment.

CellEngine software dashboard showing flow cytometry data analysis with autogating
OTS flow cytometry panel list for whole blood and cryopreserved PBMC samples

Our OTS Panels

Support your immune monitoring studies with our comprehensive list of off-the-shelf panels for whole blood and cryopreserved PBMC samples. Our OTS panels are pre-developed and validated at all global sites, providing the fastest runway to clinical sample analysis.

Key Applications

​Service ​Application
Basophil Activation ​Degranulation response assessment
Cell Enumeration MDSC and CAR-T tracking
Cellular Proliferation ​Track biological processes
Cellular Phenotyping Subpopulation evaluation
Cell Sorting (FACs) ​Achieving high cell purity
Intracellular Cytokine Staining ​Functional cytokine profiling
Receptor Occupancy ​PK/PD drug-target binding
TBNK flow cytometry assay fit-for-purpose validation and inter-laboratory cross-validation poster

Case study

Validated TBNK Assay

CellCarta conducted a fit-for-purpose, cross-validation of our enhanced TBNK assay across our global immune monitoring labs. By adding CD20 to detect B cells even when CD19 is masked by therapy, our workflow meets rigorous regulatory standards with consistent precision.

Need flow cytometry services for your clinical program?

Speak with our scientist about your study requirements and we’ll help you identify the best approach for your needs.

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Frequently Asked Questions

As a platform-agnostic laboratory, we offer a range of multiparametric flow cytometry platforms for single-cell analysis, including, BD Lyric™, BD LSRFortessa™, Cytek™ Aurora, BD FACSAria II™, and CyTOF®. Our flow cytometry services cover the full range of immune monitoring applications, including cell enumeration, cellular phenotyping, cellular proliferation, intracellular cytokine staining, receptor occupancy, basophil activation testing, and fluorescence-activated cell sorting. Both pre-validated and custom panels are available for a range of clinical sample types, including cryopreserved PBMCs, whole blood, bone marrow aspirate, and cerebrospinal fluid.

Inter-site MFI variability is one of the most common challenges in multi-center flow cytometry studies. CellCarta addresses this through lyophilized bead-based instrument alignment, which has been used to standardize more than 10 assays across five global laboratory sites, achieving coefficients of variation below 30% across inter- and intra-site readouts. Off-the-shelf panels are pre-validated at all sites to the same standard, eliminating the need for per-site revalidation.

Yes. CellCarta’s global PBMC processing network operates across laboratories in Canada, the USA, Belgium, China, and Australia, with same-day processing capabilities and robust SOPs delivering greater than 95% cell viability. Our sample management services coordinate directly with clinical sites on shipping, sample tracking, and import and export requirements, achieving on-time study setup.

Yes. CellCarta offers a broad range of complementary technologies alongside flow cytometry solutions, including ELISpot and FluoroSpot for functional immune response monitoring, mass cytometry for higher-parameter phenotyping, ELISA for soluble biomarker quantification, genomics for transcriptomic context, and spatial biology for tissue-level immune profiling.

CellCarta uses CellEngine®, our proprietary cloud-based software for rapid, scalable data analysis across our flow cytometry services. Machine learning-assisted autogating reduces manual analysis time across large datasets, while metadata-driven workflows minimize human error. Batch comparisons, longitudinal study visualizations, and advanced clustering algorithms, including UMAP and FlowSOM, run in minutes rather than hours. CellEngine® operates in a 21 CFR 11-compliant environment.