Multi-Site Instrument Alignment Using Lyophilized BD™ CompBeads

May 3, 2024

Dominic Gagnon, Kevin Lo

CellCarta’s poster presents a robust method for multi-site instrument alignment using lyophilized BD™ CompBeads to ensure consistency in flow cytometry data across global locations. This method addresses the challenge of variability in mean fluorescence intensity (MFI) readouts by utilizing lyophilized beads that are stable at room temperature for 18 months.

The study confirmed that this approach allows for reliable data transfer and alignment of flow cytometric assays across different sites, reducing operator variability and shipping needs. The method was tested for stability, precision, and accuracy, demonstrating that it can maintain consistent performance over time and across various fluorochromes.

View the poster:

Multi-Site Instrument Alignment Using Lyophilized BD™ CompBeads