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Immunohistochemistry (IHC) Biomarkers Multiplex IHC (mIHC) Biomarkers
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2018 White Paper on Recent Issues in Bioanalysis: focus on flow cytometry, gene therapy, cut points and key clarifications on BAV(Part 3 – LBA/cell-based assays: immunogenicity, biomarkers and PK assays)

Immune Monitoring

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Immunohistochemistry (IHC) Biomarkers

1-9

15PGDH
4E-BP1
5T4

A

AKT
ALK
Aurora A

B

B2-Microglobulin
B7H3
BAX
BCL2
BCL6
BCL-xL
BCMA
BIM

C

CAIX (CA9)
CCR8
CD10
CD11b
CD11c
OX40 (CD134)
CD137
CD137L
CD138
CD15
CD16
CD163
CD16a
CD1a
CD20
CD27
CD70 (CD27L)
CD28
CD3
CD30
CD31
CD34
CD37
CD38
CD39
CD4
CD40
CD45
CD56 (NCAM)
CD79b
CD68
CD73
CD8
CD80
Chromogranin A
cIAP-1
Claudin-18
Cleaved Caspase 3
CLEC9A (CD370)
c-MET
c-MYC
COX2
CSF1R
CTLA-4 (CD152)
Cyclin D1
Cyclin E1
cPARP

D

Desmin
DNAM (CD226)
DR5
DLL3

E

E-Cadherin
EGFR
ER
ERCC1
ERCC5
ERK

F

FAP
FASCIN
FASN
FGL1
FOXM1
FOXP3
Fucosyl-GM1

G

GITR
Granzyme B (GZMB)
GSK3B

H

HER2
HLA-E
HER2 (Herceptest)
HIF-1 alpha
HLA Class 1 ABC (MHCI)
HGF
HLA-DP, DQ, DR (MHCII)
HSP47
HSP70

I

ICOS
IDO
IGF1R beta
IL8

K

Ki67

L

LAG3
LDLR

M

MDM2
MEK1/2
Mesothelin
MLH1
MPO
MSH2
MSH6
MTAP
MUC13
MUM1
HLA Class 1A (MHCI)

N

Nectin-2 (CD112/PVRL2)
Neuropilin-1
NKp46 (NCR1)
NY-ESO-1

O

OX40L

P

p21
p53
panCK
panTRK
PAX5
PD-1
PD-L1 (22C3)
PD-L1 (28-8)
PD-L1 (SP142)
PD-L1 (SP263)
PD-L2
PDPN
Phospho-4EBP1 (Thr37/46)
Phospho-EGFR (Tyr1068)
Phospho-EGFR (Tyr1086)
Phospho-EGFR (Tyr1173)
Phospho-ERK (Thr202/Tyr204)
Phospho-GSK3b (Ser9/21)
Phospho-H2AX (Ser139)
Phospho-HER2 (Tyr1221/1222)
Phospho-HER3 (Tyr1289)
Phospho-HH3 (Ser10)
Phospho-MEK (Ser217/221)
Phospho-NDRG1 (Thr346)
Phospho-p90RSK (Ser377)
Phospho-PRAS40 (Thr246)
Phospho-Rb (Ser807/811)
Phospho-S6 (Ser235/236)
Phospho-S6 (Ser240/244)
Phospho-SMAD2 (Ser465/467)
Phospho-STAT3 (Tyr705)
Phospho-Yb1 (Ser102)
PI3K p110alpha
Phospho-KAP-1
PMS2
PPARγ
PR
PRAS40
PSMA
PTEN
p16
Phospho-Met (Tyr1230/Tyr1234/Tyr1235)
Phospho-Met (Tyr1349)
Phospho-mTOR (Ser2448)
Phospho-p70S6 Kinase (Thr421/Ser424)
Phospho-p70S6K (Thr389)

R

ROS1

S

S100
S6
SDMA
SRC
STING
Synaptophysin

T

TIGIT
TIM-3
TLR-7
TUNEL Assay

V

Vaccinia
Vimentin
VEGFR2
VEGFA

W

WT1

Y

Yb1

Multiplex IHC (mIHC) Biomarkers

C

CD3 + CD4 + FOXP3
CD3 + CD8
CD3 + CD8 + GZMB
CD3 + CD16 + GZMB
CD3 + CD68 + PD-L1
CD3 + H3K27me
CD3 + LAG3
CD3 + PD-1
CD4 + CD8 + FOXP3
CD4 + CD8 + ICOS
CD4 + FOXP3
CD4 + FOXP3 + Ki67
CD4 + Ki67
CD8 + CD11b + PD-L1
CD8 + CD28
CD8 + CD39
CD8 + CD138
CD8 + FOXP3
CD8 + FOXP3 + GZMB
CD8 + FOXP3 + LAG3
CD8 + FOXP3 + PD-1
CD8 + GZMB
CD8 + Ki67
CD8 + PanCK
CD8 + PD-1 + Ki67
CD8 + PD-L1
CD8 + STING
CD11c + Ki67
CD16 + CD163 + GZMB
CD20 + PAX5
CD31 + ANG-2
CD31 + aSMA
CD33 + CD34
CD33 + CD34 + H3K27me
CD34 + CD117
CD38 + CD138 + AKT
CD38 + CD138 + Cleaved caspase-3
CD38 + CD138 + Ki67
CD38 + CD138 + Phospho-AKT (Thr308)
CD38 + CD138 + Phospho-FOXO
CD38 + CD138 + Phospho-GSK3β
CD38 + CD138 + Phospho-PRAS40
CD38 + CD138 + PRAS40
CD68 + CD163
CD68 + PD-L1
CD138 + B7-H3 + DR5
CD138 + BCMA
CD138 + cMYC
CD138 + Phospho-HH3
CK5 + GATA3
CD3 + CD8 + GZMB + Ki67 + NKp46 + panCK
CD3 + CD4 + CD8 + CD68 + FoxP3 + PD-1 + PD-L1 + Sox1

I

IGF1R + InsR

K

Ki67 + Cleaved caspase-3
Ki67 + pHH3

M

MHCI + MHCII

N

NKp46 + GZMB

P

Phospho-AKT + AKT
Phospho-CDC2 + CDC2
Phospho-ERK1/2 (Thr202/Tyr204) + ERK
Phospho-p70S6 Kinase (Thr389) + p70S6 Kinase
Phospho-PRAS40 + PRAS40
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See also

Download our IHC biomarker list (PDF) Digital Pathology Pathology Team

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