Immunology

CellCarta’s Immunology Services provide comprehensive assay development and testing solutions for pharmaceutical and biotech partners.

We specialize in developing and validating advanced immune monitoring assays to support both clinical and preclinical studies, leveraging deep scientific expertise in immunology.

Our professional team and state-of-the-art platforms enable high-quality data generation, helping you monitor the immune system effectively and advance your programs in immunotherapy, vaccines, and immune-mediated diseases.

As immunology specialists, we push the field forward through continuous innovation in assay development​. We offer both fit-for-purpose assay design for exploratory, primary, or secondary endpoints.

Assays are developed with rigorous quality controls in our CAP-accredited, CLIA-certified laboratories, ensuring reliable and reproducible results. Our immunologists collaborate with you from assay concept through data analysis, providing guidance on study design, methodology, and validation to meet your specific needs​.

This consultative approach yields highly customized solutions. We don’t just provide a list of assays; we create new ones to answer your unique scientific questions​.

Why our assay expertise stands out:

• Tailored Solutions: Beyond a catalogue of assays, our team develops novel assays for challenging targets, enabling investigation of all components of the immune system – even rare events like
• Quality & Compliance: Assays are designed under strict quality management. We adhere to industry guidelines and have even helped author standard protocols (e.g., for receptor occupancy and intracellular cytokine staining) to ensure robust immunology data​
• Clinical Readiness: We bridge research and clinical practice by validating critical biomarker assays for use in patient trials. For example, our team carefully validated a 29-plex cytokine assay on the Meso Scale Discovery (MSD) platform for high-throughput clinical sample analysis​.

Flow cytometry is a cornerstone of our immunology services, and CellCarta offers unparalleled expertise in multiparametric flow cytometry for immune cell profiling. We provide qualitative and quantitative analysis of cells at the single-cell level​ delivering rich phenotypic and functional data.

Our laboratories are equipped with cutting-edge flow cytometers, including spectral flow instruments (Cytek Aurora) that can detect up to 40 parameters per cell for deep immune profiling​. This capability allows comprehensive analysis of complex cell populations in a single run.

With our flow cytometry services, you gain a detailed picture of the immune landscape, whether monitoring immune reconstitution in cell therapy, analyzing immune checkpoint modulation in oncology, or assessing immunotoxicology in preclinical models.

All flow cytometry data is generated with the level of quality and reproducibility required for confident decision-making in drug development.

Understanding soluble immune biomarkers is equally critical. CellCarta offers a suite of multiplex immunoassay platforms to quantify cytokines, chemokines, and other biomarkers with high sensitivity and precision. The results is a comprehensive view of the immune signaling environment from a small sample, ideal for detecting subtle changes or correlations with clinical outcomes.

Our immunoassay capabilities span four major platforms to suit various project needs​:

• Olink® : An ultra-high multiplex platform that can profile hundreds of proteins with exceptional sensitivity, using a minimal sample. This is powerful for broad biomarker discovery or profiling in translational research.
• MSD® : A high-sensitivity, moderate-plex platform (up to 10-plex per well, 40-plex per panel) ideal for targeted panels of cytokines, chemokines, growth factors, etc., with excellent dynamic range and quantitative performance.
• Ella™ : A next-generation automated ELISA system that runs multiple analytes simultaneously. Ella provides rapid turnaround and high precision for a smaller set of markers (e.g., 4–8 analytes) with minimal hands-on time, which is great for streamlined analysis in clinical studies.
• ELISA: A gold-standard, single-analyte immunoassay for cases where maximal specificity is needed for one biomarker. We run high-throughput ELISAs with rigorous controls to deliver robust and reproducible data.

Having these platforms in-house means we will choose the optimal assay technology for your biomarkers of interest, balancing multiplex needs, sensitivity, and sample volume.

Our team has extensive experience in ligand-binding assays, detecting proteins and antibodies even at low concentrations​.

We routinely measure cytokines, chemokines, soluble receptors, and pharmacodynamic markers of drug activity.

Benefits of our multiplex immunoassay services:

• High Throughput & Efficiency: Analyze multiple biomarkers in each sample simultaneously, reducing sample requirements and cost per data point.
• Sensitivity & Dynamic Range: Detect low-abundance immune mediators with broad dynamic ranges, capturing both subtle and strong immune responses​.
• Small Sample Volumes: Preserve precious samples – our assays often require only microliter quantities of serum, plasma, or culture supernatant​.
• Flexibility: Choose from a range of standard panels or request custom assay development to focus on the biomarkers most relevant to your study​. We tailor assays for specific cytokine profiles or disease biomarkers as needed.
• Data Quality: Obtain quantitative, publication-ready data. Our immunoassays are performed with replicates and appropriate standards/controls to ensure accuracy.

By combining cellular assays (flow cytometry, ELISpot) with soluble factor analysis (multiplex immunoassays), CellCarta delivers a complete immune profile for your samples​ giving you correlated insights into how immune cells and cytokines interact in the context of therapy or disease.

 Explore our Immunoassay Brochure to learn more about our capabilities and how we can support your study!

CellCarta’s immunology capabilities support a wide array of therapeutic areas and research applications. Our scientific team has experience applying these assays in autoimmune diseases, oncology/immuno-oncology, infectious diseases, and more.

We understand that each domain has unique immune monitoring needs, and we adapt our approach accordingly:

• Autoimmune & Inflammatory Diseases: We develop assays to measure immune dysregulation and drug mechanism of action in conditions like rheumatoid arthritis, lupus, multiple sclerosis, and others. For example, using our pathway phenotyping platform, we assessed how different JAK inhibitors suppress intracellular signaling in immune cells– data that can guide dose selection and therapeutic strategy in autoimmune disease trials. We can quantify inflammatory cytokines, track T cell subsets (e.g. Th17, Tregs), and evaluate biomarkers of disease activity or drug response.
• Oncology & Immuno-Oncology: Our services are integral to cancer immunotherapy programs, where immune monitoring is key. We perform comprehensive immune profiling in cancer patients, including characterization of peripheral immune cells during checkpoint inhibitor therapy, analysis of tumor-infiltrating lymphocytes in tumor samples​, and functional assays to gauge T cell cytotoxic responses. We support CAR-T and cell therapy trials by tracking phenotype and persistence of administered cells, as well as immune reconstitution. Our multiplex assays measure immunomodulatory cytokines (like IL-6, IFN-γ) and soluble checkpoints, providing a full picture of the immune milieu in oncology studies.
• Infectious Diseases & Vaccines: We have specialized panels and assays for evaluating immune responses to infectious disease treatments and prophylactic vaccines. Using technologies like MSD®, we can assess vaccine efficacy by measuring panels of cytokines and chemokines post-vaccination.. Our ELISPOT/FluoroSpot assays help enumerate antigen-specific T cells (for example, IFN-γ producing T cells in viral infections or vaccine trials). With experience in diseases from influenza and COVID-19 to HIV and tropical diseases, we provide immune monitoring that supports infectious disease therapeutic development.
• Translational Research & Biomarker Discovery: Whether you are in early drug discovery or pre-clinical research, our immunology services help identify and validate biomarkers. We can screen for changes in immune cell gene expression correlating with flow cytometry data, or confirm candidate biomarkers discovered via proteomics using targeted immunoassays. By integrating multi-omics data with immune readouts, we help uncover mechanistic insights and surrogate markers that drive your program forward.

In each of these areas, CellCarta’s end-to-end capabilities – from sample processing (including PBMC processing services) to complex data analysis – ensure that you have a reliable partner to interrogate the immune system from every angle.

Our breadth of experience means we can rapidly apply proven methods or develop innovative assays for novel targets, as your project requires.

CellCarta stands out as a global leader in immunology assay services for several reasons:

• Unmatched Scientific Expertise: With decades of experience in immunology research and assay development, our scientists bring a deep understanding of immune mechanisms. We have contributed to community standards (e.g., flow cytometry guidelines​) and have a strong publication record, reflecting our commitment to scientific excellence. This expertise translates into assays that are biologically relevant and technically sound.
• Integrated Platforms Under One Roof: We offer a unique integration of technologies – from high-dimensional flow cytometry to advanced multiplex immunoassays and even histopathology and genomics – all within one organization. This allows us to design comprehensive immune monitoring strategies and handle complex projects that require multiple readouts. Rather than juggling multiple vendors, you can rely on CellCarta for a cohesive solution that harmonizes data across platforms.
• Quality and Regulatory Compliance: Every service is delivered with rigorous quality assurance. Our laboratories operate under GLP/GCLP-compliant systems with CAP accrediations and CLIA certifications. We follow international guidelines and perform thorough assay validations, so you can trust the data for clinical decision-making, or regulatory submissions.
• Global Reach & Capacity: CellCarta’s global laboratory network ensures your immunology studies can be supported seamlessly around the world. We have the capacity to process large cohorts and the logistical expertise (including kit design and sample management) to maintain sample integrity from collection to analysis. Our Global PBMC Processing Services and sample logistics teams make multinational trials feasible and efficient.
• Collaborative Approach: From the early planning stages, we work closely with your team to understand the project objectives and tailor our services accordingly. Regular communication, data review meetings, and scientific input are part of our workflow, ensuring that the assays we deliver truly answer your research questions.

CellCarta provides industry-leading immunology services that accelerate your drug development and discovery programs.

Whether you need standardized immune monitoring for a clinical trial or a custom-developed assay for an exploratory endpoint, our team is ready to deliver with precision and reliability.

Contact CellCarta today to discuss our immunology assay capabilities.