Access Superior Nucleic Acid Quantification with Digital PCR Services
Our Digital PCR expertise
As an expert dPCR service provider, our team supports any dPCR-based application, using either off-the-shelf assays or including custom primer design, all performed in an ISO 13485 accredited environment.
Our team harnesses the power of both the QIAcuity’s multicolor digital PCR and the Bio-Rad QX200TM dPCR platforms,with validation across all our sites to provide support for global clinical trials.
Key applications of Digital PCR (dPCR)
dPCR enables nucleic acid quantification with superior accuracy, precision and sensitivity.
This technology excels in:
- Expression profiling of RNA molecules
- Quantification of low abundant genes or small differences
- Absolute quantification
- Gene Copy Number Variation (CNV)
- Vector Copy Number (VCN) determination and Replication Competent Retrovirus (RCR) or Lentivirus (RCL) detection, in the context of adoptive cell therapies
- Rare event detection, such as cancer gene mutations with low variant allele frequency in both tissue samples and liquid biopsies (including blood samples)
As absolute concentrations are determined, data can be easily interpreted and compared across different samples and experiments, especially in a clinical setting.
Choosing between dPCR and qPCR
Digital PCR leads to absolute quantification and does not require the use of standard curves as qPCR.
The assay particularly excels in providing the quantification of low abundant targets or small differences.
Learn more about the intrinsic potential our digital PCR (dPCR) assays in clinical studies with 5 applications performed by our team:
- Expression profiling of RNA molecules
- Rare variant analysis in cell-free DNA (cfDNA)
- Quantification of small changes in alternative splicing
- Absolute quantification of CAR T cells biodistribution
- Absolute quantification of CAR T vectors in clinical trial
At the forefront of digital PCR based research
Our expertise in dPCR is showcased in different publications:
- The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 (Huggett JF et al. Clin Chem, 2020)
- Quality control of digital PCR assays and platforms (Vynck M et al. Anal Bioanal Chem, 2017)
- Flexible analysis of digital PCR experiments using generalized linear mixed models (Vynck M et al. Biomol Detect Quantif, 2016)