Quantitative PCR (qPCR) and reverse transcription qPCR (RT-qPCR) are utilized for targeted quantification of DNA or mRNA, microRNA and lncRNA.
It is well known that qPCR-based approaches for gene expression studies have excellent sensitivity, specificity, linear dynamic range of quantification, as well as a high level of flexibility.
In our laboratories, we have processed tens of thousands of clinical samples, including various types of liquid biopsies and FFPE material using qPCR for quantification of both protein coding messenger RNAs (mRNAs) and non-coding microRNAs and long non-coding RNAs (lncRNAs).
Our team has proficiencies with the following key platforms: cobas (Roche), Rotor-Gene Q (Qiagen), Idylla (Biocartis), QuantStudio (ThermoFisher), and Promega.
Our team can support any qPCR-based application, using either off-the-shelf qPCR assay or expertly designed and validated custom primers.
Various qPCR projects performed both in research and clinical trial settings:
Our team is internationally recognized for its qPCR expertise, setting standards in gene expression analysis with landmark papers and contribution to guidelines: